Molecular Characterisation

• Determination of size and copy number of detectable inserts (complete and partial)
• Analysis of the organisation of the inserted genetic material at the insertion site
• Proof of the absence of undesired sequences
• Description of the expression of interest
• Analysis of the genetic stability of the insert
• Analysis of the concentrations of the new gene products
• Molecular characterisation of the newly expressed protein
• Search for homology to known proteins

Methods:

• Real time PCR
• Southern blot
• PCR
• Northern blot
• Real time Rt-PCR
• Western blot
• Protein quantification
• Protein activity tests